Form _'s general knowledge of gene expression of rectal cancer

Form _'s general knowledge of gene expression of rectal cancer
According to Danish scholar, form rectal cancer (CRC) Gene and expressing the array mark (EST) Change become being of preventing and curing CRC to be that target order.

Dr. Karin Birkenkamp-Demtroder in the Danish Aarhus university molecule diagnostics laboratory points out, while defining new biological mark and treatment, need to understand that form rectal cancer (CRC) Molecule change. For this reason, researchers utilize the widowed nucleotide chip, monitor the left side and distribute 5 pools (each pool include 4 to 6 samples) of all RNA which forms rectal cancer 6,800 known gene and 35,000 expression array marks (EST) of China Gene expression.

Have more normal researchers relatively tissue and Duke phased A-D stage ' Have not soaked to carry shifting far) Tumour organization,and it discern from it is normal development change by prominent 908 pieces of genes and 4,155 EST known when not organizing by tumour. Found finally, strained through strengthening except that, 226 known gene and 157 ESTs correlated with CRC heighted.

Carry on array analyze, find to 25 single sample> 70% case prove known change of gene. Divide layer connect, form a cluster, analyze, collect, form a cluster Duke A stage and normal organization separately equally and Duke B stage, and Duke C stage collect form a cluster and Duke D stage collect form a cluster bidirectionally. 10 known gene and real-time PCR of 5 ESTs reveal, the discovery based on array has good repetitiveness. Change the intersection of frequency and supreme the intersection of gene and supersession of lying in ' 22%) , duplicate and translate ' 11%) Punish with cell ' 9%) Function area. At the CRC, 15 nuclear mitochondrial albumen of code all lower and regulate.

Researchers have discerned that have possible cancer gene or a plurality of chromosome areas where tumour inhibits the gene from forming a cluster. Some of them, such as aminopeptidase N/CD13 and sigma B3 albumen on 15q25 chromosome, and send and mix and shut the something small and hard to lose compatibly frequently.

nbspBirkenkamp-Demtroder doctor thinks, gene and EST that should discover can encode the new possible tumour mark, and can become the new treatment goal of preventing and curing CRC.


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