Analyze _'s male sterility of accessory system of the computer in sperm's energy

Analyze _'s male sterility of accessory system of the computer in sperm's energy
First, summary
Relevant sperm's density in recent years, the automatic analyticl system of the characteristic of activity force and movement is known as the computer to assist the seminal fluid analyticl system (computer-assisted semen analysis, CASA) Has already employed extensively at home and abroad, a frontier studied that thus open man sexology. In order to distinguish semen analysis and sperm's special analysis of the characteristic of moving, the latter is known as computer assisting or the computer automatic sperm's energy analyticl system (computerassisted again, orcomputer-automated, spermmotilityanalysis, CASMA) ,Because CASMA might not be connected with semen analysis. But the intension of this kind of term is not very clear, especially ignore some other situations, for instance: In study human sperm (by the intersection of sperm and density and the intersection of activity and the intersection of sperm and percentage that wash) with CASA under being the intersection of semen analysis and terms ; CASA is used in the non-human sperm to measure under condition of any observation; Analyze the other respects of the sperm, such as morphology.
Already there is commercial seminal fluid analyticl system of sperm's energy in U.S.A., mainly there are the following aspects in earlier stage: CellSoft (2000 sum of system 3000),HT-M2030 and CTS-30 system. In addition, there are VICOS-SPERM which Germany produces and LABSCAN VI which U.S.A. produced. There are some analyticl systems of researching and developing besides, will introduce to the market soon too. CASA the intersection of CASMA and system (if digital picture analyze) The basic principle operated is the same, but in optical systems used, picture obtain technology and the intersection of sperm and recognition technology, locus rebuild computing technology, sport analyze computing technology and data analysis respect exist greater difference all. Obviously, should use these methods or utilize a method among them to get the data, must all be familiar with the basic principle of sperm movement analyzing and special analysis. This will done some and discussed with the difference to some more important characteristics. The user should also read users and guide the manual conscientiously, and must remember software and even hardware of these systems will often also have obvious changes and improvements.
Second, influence that the sperm collides with
If two sperms moving about collide each other, will interfere with their own sports, its influence is very prominent. Likewise, move about sperm and move sperm, bigger the intersection of sperm and chip or other cells (such as the leucocyte) Collide, will produce the similar consequence too. In fact, because the swing range of the sperm tail is as wide as orbit measured by ALH at least, change the collision that the sports of the sperm might not take place in fact. A problem difficult to solve while analyzing by a sport. In Cellsoft system, acts as the biggest likelihood ring (circle ofmaximun likelihood) of an object ,Namely the area of the next possible position, when include being discerned as the second object of the son's, Cellsoft will stop following the trail of this object, the maximum speed that the radius reliance of this ring presumes in Cellsoft parameter, it is basically the distance of maximum expectation that the sperm can be moved among picture of the camera system. If the orbits of two bodies in motion overlap, then the two reject in analyzing. This avoids causing a safe method of the possibility out of shape of the orbit because the sperm collides. If the orbit is too short ' What presume is required the most low in this systematic parameter 15 pictures or 0.5s) ,Can't do the movement characteristic analysis of the sample. HT-M2030 and CTS-30 system does not repel colliding, thus difficult to influence by sperm's density (see after statedly) . Whether the average sport characteristic of its sample of sperm has got rid of obvious simply resting on, still remain to further verify. Generally speaking, we do not still know the actual influence of colliding to the movement characteristic of the single sperm.
Third, density dependence
An important problem of Cellsoft systematic orbit computing technology is affected greatly by density. Best the intersection of sperm and sport analyze, can only suitable for precise kinds of little on 40* 106 sperm seminal fluid of mL density, and presume it is 200 m s that the speed is the highest. The intersection of seminal fluid and density will cause orbit in missing possibility increase under analytic process of sport while being too high, and is not only influenced by the fact that the influencing of sperm's density is also distributed by activity sperm's proportion and speed. If presume the too high speed the most greatly, for example to the sperm (usually 300- 400 m s) that is washed Or it is too deep to prepare liquid, this system can do exercises the largest sperm's density of analyzing will be reduced.
Fourth, trader use CASA CASM A system
(1) Cellsoft2000
Cellsoft2000 one person who simplify and practical Cellsoft3000 basically, with appropriate to spend clinical practice, its basic seminal fluid characteristic( Such as sperm's density and energy analysis) The function is the same as Cellsoft3000, but the latter can also offer qualitatively ago the sport speed. Because the characteristic index that Cellsoft2000 can't offer sperms to move, can be thought this system to only have CASA function and not have CASMA function. Cellsoft2000, as a kind of seminal fluid automatic analyticl system, its accuracy and precision are the same as Cellsoft3000.
(2) Cellsoft3000
1.The basic structure of the basic function Cellsoft3000 includes: One is installed in Olympus (Olympus) The black-and-white camera on BH2-S microscope, 2 video monitors (one is used for revealing the true video figure, another one is used for carrying on number treatment to the picture) ,A choice VCR, a 386 computers (propose mixing another processor of association of Taiwan) of one With a lattice printer. Heatable the intersection of microscope and year the intersection of thing and platform take exercises, it analyses and researches to be essential too to sperm.
Have already used Makler and PetroffHausser to count the pool and ordinary glass slice of year of microscope successfully on Cellsoft3000, but while using Horwell to count the pool, because its bottom of base is too thick, the picture obtained is disappointing.
This system has CASA and CASMA double function, can be suitable for the camera being suitable for the video cassette recorder. Though already there is this systematic parameter recommended using at present, but advise every user to determine one's own best settlement by comparing with standard seminal fluid analytical method ' See section six of this chapter) .
|